Application of OEM peristaltic pumps in laboratory small fermenter fermentation experiments

It is generally believed that the fermentation tank below 500L is a laboratory-scale fermenter, and the fermenter above 500L is a production-scale fermenter. The small fermenter fermentation in the laboratory is the prototype and foundation of industrial fermentation tank fermentation, providing data information for the operation of fermentation tanks on a production scale, including: seed validation, media formulation screening, optimization of process parameters, etc. Therefore, doing a good job of fermentation in small fermentation tanks in the laboratory is the only way to put into industrial production in the future!crgRongbai Pump

Laboratory-scale fermenters are generally equipped with four-way peristaltic pumps for each reaction vessel, generally using fixed-speed peristaltic pump OEM products, and the control system controls the addition amount by controlling the start and stop time of the peristaltic pump, and the typical product is OEM 03+TH15.crgRongbai Pump

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Among them, two peristaltic pumps add acid and alkali: the control system controls the pH value of the reaction environment at the set target value through PH value monitoring and closed-loop control of adding acid or alkali.crgRongbai Pump

One peristaltic pump with defoamer: The control system controls the amount of foam in the reaction environment to the set target value through foam monitoring and closed-loop control of adding defoamer.crgRongbai Pump

The other peristaltic pump adds medium: the control system controls the nutrient level of the reaction environment at the set target value through the closed-loop control of nutrient level monitoring and the addition of nutrient reagents.crgRongbai Pump

In fermentation tank fermentation tests, the fermentation medium is used for the growth, propagation and synthesis of products by strains. It not only makes the seeds grow rapidly after inoculation to reach a certain mycelial concentration, but also enables the grown bacteria to quickly synthesize the required products. Therefore, it is required that the composition of fermentation medium should be rich and complete, and carbon and nitrogen sources should pay attention to the combination of fast-acting and late-acting, and use less fast-acting nutrients and add more late-acting nutrients; Also consider the appropriate carbon-nitrogen ratio and add buffer to stabilize the pH; In addition, there are also growth factors and elements, precursors and accelerators required for the synthesis of growth and products. In addition to the elements and compounds necessary for the growth of the bacterium, there are also specific elements, precursors and accelerators required for the product. However, if the concentration of total carbon, nitrogen, phosphorus, etc. required for growth and biosynthetic products is too high, or the optimal conditions required by the two stages of growth and synthesis are different, the medium can be considered to be fed in batches.crgRongbai Pump

Reagents such as defoamers are also essential, because when performing fermentation experiments, microorganisms must produce metabolites, which will cause changes in pH in the bacterial liquid, and as the fermentation progresses, a large number of bubbles will definitely be produced, so that the dissolved oxygen of the bacterial liquid will decrease, resulting in the inability of the bacteria to grow and reproduce normally. At this time, acid and alkali are needed to adjust its pH, and a defoamer is used to eliminate a large number of bubbles to achieve the most suitable growth environment.crgRongbai Pump